An Integrative Analysis of The Micro-RNAs Contributing in
Stemness, Metastasis and B-Raf Pathways in Malignant
Melanoma and Melanoma Stem Cell
Epithelial-mesenchymal transition (EMT) and the stemness potency in association with
Materials and Methods
In this experimental study, 18 miRNAs that importantly contribute to EMT and have a role in regulating self-renewal and the BRAF pathway were selected based on current literature and cross-analysis with available databases. Subsequently, their expression patterns were evaluated in 20 melanoma patients, normal tissues, serum from patients and control subjects, and melanospheres. Pattern discovery and integrative regulatory network analysis were used to find the most important miRNAs in melanoma progression.
Among 18 selected miRNAs, miR-205, -141, -203, -15b, and -9 were differentially expressed in tumor samples
than normal tissues. Among them, miR-205, -15b, and -9 significantly expressed in serum samples and healthy donors.
Attribute Weighting and decision trees (DT) analysis presented evidence that the combination of miR-205, -203, -9, and
-15b can regulate self-renewal and EMT process, by affecting
We suggested here that miR-205, -15b, -203, -9 pattern as the key miRNAs linked to melanoma status, the pluripotency, proliferation, and motility of malignant cells. However, further investigations are required to find the mechanisms underlying the combinatory effects of the above mentioned miRNAs.