Neonatal Maternal Separation Alters Gelatinase Activity in Mouse
Ovarian Preantral Follicles
The early life environment is critical for normal growth and development for future reproductive function. This study aims to investigate the effect of neonatal maternal separation (MS) on gelatinase activity of mouse ovarian follicles.
Materials and Methods
In this experimental study, infants from female NMRI mice were randomly allocated into two
groups immediately after birth: i. MS isolated from their mothers for 6 hours per day, from postpartum days 2 to 16) and
ii. Control (undisturbed during the 16 days). Ovarian tissues were dissected to perform differential counts of the ovarian
follicle type by haematoxylin and eosin staining. The isolated follicles were cultured for 12 days. Gelatinase activity and
the gene expressions of matrix metalloproteinases,
Follicle counts at the different developmental stages were significantly different between the control and MS
groups. There was a significant decrease in gelatinase activity in the MS group compared to the control group. The
MS group showed significantly decreased gene expression levels of
MS is a stressor agent that compromises ovarian follicle development, at least via disruption of gelatinase activity and its related gene expressions.