Neonatal Maternal Separation Alters Gelatinase Activity in Mouse Ovarian Preantral Follicles

(Pages: 750-755)
Zeynab Asghari, M.Sc, 1Saeed Zavareh, Ph.D, 1,2,*Taghi Lashkarbolouki, Ph.D, 1,2Mahmoud Elahdadi, Ph.D, 1,2Behnoush Mehdizadeh, M.D, 2
School of Biology, Damghan University, Damghan, Iran
Institute of Biological Sciences, Damghan University, Damghan, Iran
Department of Pathology, Mashhad University of Medical Sciences, Mashhad, Iran
School of Biology, Damghan University, Damghan, Iran
Institute of Biological Sciences, Damghan University, Damghan, Iran
Department of Pathology, Mashhad University of Medical Sciences, Mashhad, Iran
*Corresponding Address: P.O.Box: 36716-41167 School of Biology Damghan University Damghan Iran Email:zavareh.s@du.ac.ir
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Asghari Zeynab, Zavareh Saeed, Lashkarbolouki Taghi, Elahdadi Mahmoud, Mehdizadeh Behnoush. Neonatal Maternal Separation Alters Gelatinase Activity in Mouse Ovarian Preantral Follicles. Cell J. 2021; 23(7): 750-755.

Abstract

Objective

The early life environment is critical for normal growth and development for future reproductive function. This study aims to investigate the effect of neonatal maternal separation (MS) on gelatinase activity of mouse ovarian follicles.

Materials and Methods

In this experimental study, infants from female NMRI mice were randomly allocated into two groups immediately after birth: i. MS isolated from their mothers for 6 hours per day, from postpartum days 2 to 16) and ii. Control (undisturbed during the 16 days). Ovarian tissues were dissected to perform differential counts of the ovarian follicle type by haematoxylin and eosin staining. The isolated follicles were cultured for 12 days. Gelatinase activity and the gene expressions of matrix metalloproteinases, MMP2 and MMP9, and their tissue inhibitors, TIMP1 and TIMP2, were evaluated by zymography and real-time polymerase chain reaction (PCR), respectively.

Results

Follicle counts at the different developmental stages were significantly different between the control and MS groups. There was a significant decrease in gelatinase activity in the MS group compared to the control group. The MS group showed significantly decreased gene expression levels of MMP2 and MMP9 compared to the control group. In contrast, the gene expression levels of TIMP1 and TIMP2 significantly increased in the MS group compared to the control group.

Conclusion

MS is a stressor agent that compromises ovarian follicle development, at least via disruption of gelatinase activity and its related gene expressions.