Down-Regulated Expression of Cystathionine β-Synthase and Cystathionine γ-Lyase in Varicocele, and Infertile Men: A Case-Control Study

(Pages: 176-181)
Fahimeh Akbarian, M.Sc, 1Marziyeh Tavalaee, Ph.D, 1Maurizio Dattilio, M.D, 2,*Mohammad Hossein Nasr-Esfahani, Ph.D., 1,3,*
Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
R&D Department, Parthenogen, Piazza Indipendenza 11, Lugano 6900, Switzerland
Isfahan Fertility and Infertility Center, Isfahan, Iran
Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
R&D Department, Parthenogen, Piazza Indipendenza 11, Lugano 6900, Switzerland
Isfahan Fertility and Infertility Center, Isfahan, Iran
*Corresponding Addresses: R&D Department Parthenogen Piazza Indipendenza 11 Lugano 6900 Switzerland P.O.Box: 8165131378 Department of Animal Biotechnology Reproductive Biomedicine Research Center Royan Institute for Biotechnology ACECR Isfahan Iran Emails:maurizio.dattilo@ parthenogen.ch,mh.nasr-esfahani@royaninstitute.org
Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited. This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial 3.0 (CC BY-NC 3.0) License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Akbarian Fahimeh, Tavalaee Marziyeh, Dattilio Maurizio, Nasr-Esfahani Mohammad Hossein. Down-Regulated Expression of Cystathionine β-Synthase and Cystathionine γ-Lyase in Varicocele, and Infertile Men: A Case-Control Study . Cell J. 2022; 24(4): 176-181.

Abstract

Objective

Cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE) are two important enzymes involved in One-Carbon metabolism. These enzymes play important roles in modulating oxidative stress and inflammation in male factor infertility through participating in the synthesis of glutathione (GSH) antioxidants in the trans-sulfuration pathway. Besides, the direct release of hydrogen sulfide (H2S) has anti-inflammatory and antioxidant effects. Therefore, the expression of CBS and CSE genes at mRNA levels in infertile and varicocele men was evaluated and compared to the healthy counterparts to clarify their possible role in the pathology of male infertility.

Materials and Methods

In this case-control study, semen parameter assessment (concentration, morphology, and motility of sperms) was performed on 28 men with varicocele, 43 infertile men with abnormal sperm parameters, and 19 fertile men. RNA was extracted from sperm samples followed by cDNA synthesis and real-time polymerase chain reaction (PCR) using CBS, CSE, and GAPDH primers.

Results

Sperm concentration and motility in infertile and varicocele groups were significantly lower (P=0.001), while spermatoza normal morphology was higher than fertile group (P=0.05). The expression levels of both CBS and CSE genes in infertile (P=0.04 and P=0.037 respectively) and varicocele (P=0.01 and P=0.046 respectively) groups were significantly lower than fertile group. Additionally, CBS gene expression indicated a positive correlation with expression of CSE gene (r=0.296, P=0.025) and sperm parameters.

Conclusion

In light of our findings, there is a valid rationale to consider the primary role of CBS and CSE enzymes impairment in male factor infertility which specifically may point to a deficit in the release of essential antioxidants including the H2S as a molecular basis of infertility and warrants further investigation.