Ps-19: The Programmed Cell Monocyte Origin (Pages: 28-28)

Beikzadeh B *, Delirezh N ,


Objective: The ultimate goal of regenerative medicine is to replace lost or damaged cells. Humans have a limited capacity to regenerate and restore their tissues and organs (such as blood and the liver). The use of stem cells to replace or repair damaged cells and tissues is considered, but the plasticity of adult stem cells is controversial and on the other hand these cells are a rare population with restricted potential to differentiate. Also the use of these cells is limited because paucity of specific markers, difficult propagation in culture and requiring a long period of the time until sufficient cells are obtained. Recent studies show that peripheral blood monocytes have stem cell-like features. We evaluated the potential of dedifferentiation and proliferation capacity of human peripheral blood monocytes in the presence of IL-3 and M-CSF to convert into programmed cell monocyte origin (PCMO). Materials and Methods: Peripheral blood mononuclear cells (PBMC) were isolated by using Ficoll/Hypaque. Then monocyte isolated by plastic adherence technique. The Monocytes were cultured with M-CSF and IL-3 for 6 days. Then morphology, phenotype and proliferation of PCMOs by Light microscopy, Immunocytochimestry- Flowcytometry and MTT assay respectively were analyzed. Results: We found that the monocyte in the presence of IL-3 and M-CSF cells became confluent, which was the result of both an increase in cell size and proliferation. Phenotype analyses, performed at 6th, indicated that CD45, CD34, CD177, CD15 were up-regulated after 6 days and other markers (CD68, CD1a, CD14) remained low. Conclusion: We conclude that the monocyte dedifferentiated, proliferated and become reprogrammable in the presence of IL-3 and M-CSF. These cells can be re differentiate or even switch to another cell type for use in regenerative medicine.