Chromosome Abnormalities and Viability of Vitrified
Eight-Cell Mouse Embryos at Presence of Two Different
Cryoprotectants at Different Storage Durations
Experiments were conducted to find the differences between post-thaw viability and chromosome aberrations in eight-cell mouse embryos at presence of dimethyl sulfoxide (DMSO) and 1, 2-propanediol (PROH) as croprotectants in different storage durations.
Materials and Methods:
In this case-control study, a total number of 720 mouse embryos
from about 250 NMRI mice were vitrified with 30% PROH or DMSO; each diluted
with a solution containing 30% ficol plus 0.5 M sucrose. Embryos were exposed to the
solutions for 0.5 minute at 25℃ followed by cooling in liquid nitrogen, then after appropriate
storage duration, they were rapidly warmed. Besides, there were 100 mouse embryos
for each cryoprotectant group (totally 200 embryos) as control. Embryo survival
was assessed by
The proportion of mitotic abnormalities in PROH/DMSO vitrified embryos was significantly higher than unfrozen control group. This was confirmed also by a reduced viability of the embryos as judged by a culture at the blastocyst stage (p<0.05 in all test groups).
It can be deduced that long term cryopreservation may result in chromosomal abnormalities and/or low viability.