Cloning, Expression and Functional Characterization of In-House Prepared Human Basic Fibroblast Growth Factor

(Pages: 282-291)
Hassan Rassouli, M.Sc., 1Mohammad Sharif Tabe Bordbar, M.Sc., 1Mehran Rezaei Larijani, B.SC., 2Mohammad Pakzad, M.Sc., 2Hossein Baharvand, Ph.D., 2,3,*Ghasem Hosseini Salekdeh, Ph.D., 1,*
1. Department of Molecular Systems Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
2. Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
3. Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran
1. Department of Molecular Systems Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
2. Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
3. Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran
* Corresponding Address: P.O.Box: 16635-148 Department of Stem Cells and Developmental Biology at Cell Science Research Center Royan Institute for Stem Cell Biology and Technology ACECR TehranIran Email:baharvand@royaninstitute.org P.O.Box: 16635-148 Department of Molecular Systems Biology at Cell Science Research Center Royan Institute for Stem Cell Biology and Technology ACECR TehranIran Email:salekdeh@royaninstitute.org
Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Rassouli Hassan, Tabe Bordbar Mohammad Sharif, Rezaei Larijani Mehran, Pakzad Mohammad, Baharvand Hossein, Hosseini Salekdeh Ghasem. Cloning, Expression and Functional Characterization of In-House Prepared Human Basic Fibroblast Growth Factor. Cell J. 2013; 14(4): 282-291.

Abstract

Objective:

Human basic fibroblast growth factor (bFGF) plays an important role in cellular proliferation, embryonic development, and angiogenesis as well as in several signaling pathways of various cell types. bFGF is an essential growth factor for the maintenance of undifferentiated human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs).

Materials and Methods:

In this experimental study, we present a straightforward method to produce biologically active recombinant human bFGF protein in E. coli that has long-term storage ability.

Results:

This procedure provides a rapid, cost effective purification of a soluble human bFGF protein that is biologically active and functional as measured in hESCs and hiPSCs in vitro and in vivo.

Conclusion:

The results show no significant difference in function between our in-house produced and commercialized bFGF.