Evaluation of The Number of CD4+ CD25+ FoxP3+ Treg
Cells in Normal Mice Exposed to AFB1 and Treated with
Aged Garlic Extract
Aflatoxin B1 (AFB1) suppresses the immune system. To decrease such suppressive
effects on the immune system, a wide range of herbal medicines like garlic are
utilized. Biological activities of garlic
Materials and Methods:
In this experimental research, AFB1 was separated from Aspergillus
flavus (PTCC 5004) by HPLC and AGE prepared using the Mantis method. The
Delayed-Type Hypersensitivity (DTH) test was carried out to determinate the effectiveness
of different doses of AGE and AFB1, which can both have an effect on the immune system.
Subsequent experiments were carried out on 20 Balb/c mice to estimate the effects
of AGE and AFB1 on the number of
The findings reveal that AGE increased the level of IFN-λ and IL-4 cytokines produced
by splenocytes stimulated by specific tumor antigen and decreased the number of
This study indicated that AGE is able to alter the cytokine production in normal mice into a Th1 protective pattern which is beneficial to the immune system in general and anti-tumor immunity in particular. AFB1 is able to alter the cytokine production into a Th2 protective pattern. Therefore, AGE might be used as herbal medicine with few side effects as compared to chemotherapy in treating cancers caused by substances like AFB1.