Purif ied Protein Fraction of Garlic Extract Modulates Cellular Immune Response against Breast Transplanted Tumors in BALB/c Mice Model

(Pages: 65-75)
Marzieh Ebrahimi, Ph.D., 1,2,*Zuhair Mohammad Hassan, Ph.D., 1,*Ali Mostafaie, Ph.D., 3Narges Zare Mehrjardi, M.Sc., 1,2Tooba Ghazanfari, Ph.D., 4
Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Department of Regenerative Biomedicine at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
Immunoregulation Research Center, Shahed University, Tehran, Iran
Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Department of Regenerative Biomedicine at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
Immunoregulation Research Center, Shahed University, Tehran, Iran
* Corresponding Address: P.O.Box: 193954644 Department of Immunology School of Medical Sciences Tarbiat Modares University TehranIran Email: hasan_zm@modares.ac.ir
P.O.Box: 16635-148 Department of Regenerative Biomedicine at Cell Science Research Center Royan Institute for Stem Cell Biology and Technology ACECR TehranIran Email: mebrahimi@royaninstitute.org
Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Ebrahimi Marzieh, Mohammad Hassan Zuhair, Mostafaie Ali, Zare Mehrjardi Narges, Ghazanfari Tooba. Purif ied Protein Fraction of Garlic Extract Modulates Cellular Immune Response against Breast Transplanted Tumors in BALB/c Mice Model. Cell J. 2013; 15(1): 65-75.

Abstract

Objective:

Garlic (Allium sativum) has anti-inflammatory, anti-mutagenesis, and immunomodulatory properties that modulate anti-tumor immunity and inhibit tumor growth. In this study we have examined the effect of a protein fraction isolated from fresh garlic on anti-tumor response and intra-tumor lymphocyte infiltration.

Materials and Methods:

In this experimental study a protein fraction was purified from fresh garlic bulbs using ultra-filtration, followed by chromatofocusing, and SDS-PAGE analysis. Anti-tumor activity was assessed by intra-tumor injection of the protein fraction and garlic extract, itself, into groups of 5 mice each. The percentage of peripheral blood and intra-tumor CD4+ and CD8+ cells were assessed by flow cytometry. Unpaired student’s t test using the SPSS program was applied for all statistical analyses.

Results:

Garlic extract included different type of proteins with different molecular weight. One of protein’s fraction was immunomodeulator and was composed of three single polypeptides, with molecular masses of ~10-13 kDa and different isoelectric points (pI). These molecules augmented the delayed type hypersensitivity (DTH) response compared to the control group. Intra-tumor injection of the fraction provoked a significant increase in the CD8+ subpopulation of T-lymphocytes, as well as a decrease in tumor size. The fraction increased peripheral blood CD8+ T-lymphocytes in treated animals.

Conclusion:

The data confirms that protein fractions purified from fresh garlic bulbs augment CD8+ T-cell infiltration into the tumor site, inhibiting tumor growth more efficiently than garlic extract. These findings provide a basis for further investigations on the purified polypeptide as a useful candidate for immunomodulation and tumor treatment.