Stem Cells from Human Exfoliated Deciduous Tooth Exhibit Stromal-Derived Inducing Activity and Lead to Generation of Neural Crest Cells from Human Embryonic Stem Cells

(Pages: 140-142)
Khadijeh Karbalaie, M.Sc, 1Somayyeh Tanhaei, M.Sc, 2Farzaneh Rabiei, M.Sc, 2Abbas Kiani-Esfahani, M.Sc, 2Najmeh Sadat Masoudi, M.Sc., 3Mohammad Hossein Nasr-Esfahani, Ph.D., 1,*Hossein Baharvand, Ph.D., 4,5,*
Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
Department of Molecular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
Department of Genetics at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran
Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
Department of Molecular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
Department of Genetics at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran
- *Corresponding Addresses: P.O. Box: 8165131378 Department of Cellular Biotechnology at Cell Science Research Center Royan Institute for Biotechnology ACECR Isfahan Iran Email:mh.nasr-esfahani@RoyanInstitute.org P.O. Box: 16635-148 Department of Stem Cells and Developmental Biology at Cell Science Research Center Royan Institute for Stem Cell Biology and Technology ACECR Tehran Iran Email:Baharvand@RoyanInstitute.org
Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Karbalaie Khadijeh , Tanhaei Somayyeh, Rabiei Farzaneh, Kiani-Esfahani Abbas, Masoudi Najmeh Sadat, Nasr-Esfahani Mohammad Hossein, Baharvand Hossein. Stem Cells from Human Exfoliated Deciduous Tooth Exhibit Stromal-Derived Inducing Activity and Lead to Generation of Neural Crest Cells from Human Embryonic Stem Cells . Cell J. 2021; 23(1): 140-142.

Abstract

In this article which was published in Cell J, Vol 17, No 1, Spring 2015, on pages 37-48, we found that Figure 1H, Figure 2 (OTX2, row 3), and Figure 3 (row 4) had been published incorrectly. The following figures are corrected.

The authors would like to apologies for any inconvenience caused.

- Induction of human embryonic stem cells (hESCs) into neural cells by co-culture with stromal stem cells from human exfoliated deciduous teeth (SHED). A. Stereo photomicrographs of hESC colonies with central crater-like structures, B. Numerous neural tube-like structures located in the margin of the colonies on day 14, C. Neural progenitor cells (NPCs) with rosette-like structures before passaging, D. were ZO1 (epithelial marker) positive , E. Adherent culture of NPCs , F. was NESTIN positive, G. NPCs showed normal karyotype and H. expressed NESTIN, SOX1 and PAX6.
- Immunofluorescence staining of human embryonic stem cell derived neural precursor cells (hESC-NPCs). Rostral identity and proliferation potency showed by immuno co-staining for NESTIN/OTX2, NESTIN/PAX6 and OTX2/Ki67. Neuroepithelial and radial glia characteristic demonstrated by RC2/SOX1 immuno co-staining.
- Phase contrast (A-D) and immunofluorescence staining (E) of differentiated human embryonic stem cell derived neural progenitor cells (hESC-NPCs). Visible network structures appeared following a long culture period of neural cells that had bipolar morphology and distinct soma. The differentiated cells were positive for TUJ, NCAM mature neural markers and TH, PERIPHERIN and BRN3A as markers of peripheral neurons.